uracil 5 monophosphate trisodium chem impex Search Results


uracil 5 monophosphate trisodium chem impex  (Chem Impex International)
96
Chem Impex International uracil 5 monophosphate trisodium chem impex
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
Uracil 5 Monophosphate Trisodium Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uracil 5 monophosphate trisodium chem impex/product/Chem Impex International
Average 96 stars, based on 1 article reviews
uracil 5 monophosphate trisodium chem impex - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
5 fluorouracil 42a  (Chem Impex International)
95
Chem Impex International 5 fluorouracil 42a
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
5 Fluorouracil 42a, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 fluorouracil 42a/product/Chem Impex International
Average 95 stars, based on 1 article reviews
5 fluorouracil 42a - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
iodo  (Chem Impex International)
95
Chem Impex International iodo
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
Iodo, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iodo/product/Chem Impex International
Average 95 stars, based on 1 article reviews
iodo - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
5 methyl uracil thymine  (Chem Impex International)
96
Chem Impex International 5 methyl uracil thymine
(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.
5 Methyl Uracil Thymine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5 methyl uracil thymine/product/Chem Impex International
Average 96 stars, based on 1 article reviews
5 methyl uracil thymine - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
5,6-dimethyluracil  (Chem Impex International)
N/A
   Buy from Supplier
5-aminouracil  (Chem Impex International)
N/A
   Buy from Supplier
5-chlorouracil  (Chem Impex International)
N/A
   Buy from Supplier
5-trifluoromethyluracil  (Chem Impex International)
N/A
   Buy from Supplier
5,6-diamino-2,4-dihydroxypyrimidine sulfate dihydrate  (Chem Impex International)
N/A
   Buy from Supplier
5-cyanouracil  (Chem Impex International)
N/A
   Buy from Supplier
5-fluoro-1-(tetrahydro-2-furfuryl)uracil  (Chem Impex International)
N/A
   Buy from Supplier
2,4-dihydroxy-5-methoxypyrimidine  (Chem Impex International)
N/A
   Buy from Supplier

Image Search Results


(A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Journal: bioRxiv

Article Title: TOR coordinates nucleotide availability with ribosome biogenesis in plants

doi: 10.1101/2020.01.30.927418

Figure Lengend Snippet: (A) Seedlings were treated with 0.1 μM MTX and collected over a time course, as indicated. TOR activity decrease 3 h after MTX treatment. This experiment was repeated three times; representative results are shown here. (B,C,D) Seedlings were pre-treated with 0.1 μM MTX for 3.5 h, and then supplied either inosine 5’-monophosphate (IMP) (B) , uracil 5’-monophosphate (UMP) (C) , or a mixture of nucleotide monophosphates (NMPs) (D) at physiologically-relevant concentrations (0.5, 1.0, or 2.0 mM). NMPs restored S6K-pT449 levels and S6K-pT449/S6K ratios in MTX-treated seedlings, suggesting that the effects of MTX on TOR activity are due to nucleotide depletion. Neither IMP nor UMP was sufficient to restore TOR activity, indicating that all nucleotides (purines and pyrimidines) are required to stimulate TOR in MTX-treated seedlings. This experiment was repeated five times; representative results are shown here.

Article Snippet: Methotrexate (Gainger), 6-mercaptopurine (Fisher Scientific), 5-fluorouracil (Fisher Scientific), 5-fluoroorotic acid (Fisher Scientific), adenosine-5′-monophosphate disodium (Fisher Scientific), cytidine-5′-monophosphate disodium (Chem-Impex), guanosine-5′-monophosphate disodium (Chem-Impex), inosine-5’-monophosphate (TGI), and uracil 5′-monophosphate trisodium (Chem-Impex) were prepared fresh in concentrated stock solutions that were then diluted in ½ MS media (Caisson Labs), pH = 5.7 with KOH, for application to seedlings.

Techniques: Activity Assay